1. Introduction
The right tools ensure reproducibility, sterility, and healthy plant growth. Whether you’re building a high-end lab or starting in a small room, these tools form the foundation of any tissue culture operation.
2. Core Equipment & Their Functions
| Tool/Device | Purpose |
|---|---|
| Autoclave/Pressure Cooker | Sterilizing media, tools, and vessels |
| Laminar Flow Hood | Creating a sterile air environment |
| pH Meter | Adjusting media acidity |
| Hot Plate with Stirrer | Even mixing of culture media |
| Digital Scale | Measuring ingredients accurately |
📌 Intro to Plant Tissue Culture Equipment (Source)
3. Cutting and Handling Tools
Precision and cleanliness are key. Always autoclave or flame-sterilize tools between uses.
| Tool | Use |
|---|---|
| Scalpel | Cutting tissue cleanly |
| Forceps | Handling small explants |
| Scissors | Trimming shoots or roots |
| Needles | Lifting delicate callus |
4. Culture Vessels and Containers
Important qualities:
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Transparent
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Heat-resistant
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Easy to seal
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Reusable or disposable
Different cultures require different vessels:
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Petri dishes for initial callus formation
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Magenta boxes for shoot multiplication
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Jars or flasks for rooting or full plantlet development
5. Sterile Accessories and Lab Support Items
These small items are vital for maintaining aseptic technique:
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70% Alcohol Spray Bottles – Disinfect surfaces/tools
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Parafilm – Seals containers
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Graduated Cylinders – Measuring liquids
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Labels & Lab Tape – Marking culture type, date, and stage
Note: Label clearly and consistently to avoid errors.
6. Budgeting and Alternatives for New Labs
| Lab Tool | DIY Alternative |
|---|---|
| Autoclave | Pressure cooker |
| Laminar Flow Hood | Still-air box (storage bin w/ arm holes) |
| Light system | Inexpensive LED grow lights |
| Glass containers | Baby food jars or recycled glass |
| Stirrer | Manual stirring + heat plate |
📌 Affordable Tissue Culture Lab Design