1. Overview

A tissue culture lab must be designed for:

• Sterility

• Functionality

• Safety

• Scalability

While labs can range from DIY setups in spare rooms to industrial facilities, they all share a need for organized zones, airflow control, and reliable utilities.

2. Core Functional Areas of a Lab

Each space must be physically separated or well-managed to prevent contamination.

3. Environmental Requirements

• Temperature: 24–28°C promotes optimal enzymatic activity and culture health.

• Light: ~2000 lux using cool white or full-spectrum LED lights for photosynthesis and morphogenesis.

• Humidity: 50–70% is ideal. Too much moisture leads to fungal growth; too little causes desiccation.

• Air Filtration: Laminar flow hoods with HEPA filters are essential for sterile work.

📌 Source – Growth Environment for Tissue Culture

4. Utilities & Infrastructure

Must-have utilities include:

• Distilled or deionized water

• Reliable electrical connections

• Stable benchtops and flooring

• Access to waste disposal and emergency services

Bonus Tip: A backup power supply protects against power loss during critical growth periods.

📌 Setting Up a Tissue Culture Lab – Practical Guide

5. Safety and Workflow

A well-organized lab prioritizes workflow and safety.

• Always work from clean → dirty areas

• Install eye wash stations, fume hoods, and PPE stations

• Flammable chemicals should be stored in flameproof cabinets

• Minimize dust and foot traffic in sterile zones

📌 Biosafety Guidelines in Lab Design

6. Budget Considerations for Beginners

For home or classroom setups:

• Use converted rooms with tables and adjustable lighting.

• Replace autoclaves with pressure cookers.

• Use alcohol-based sterilization where autoclaving isn’t possible.

• Laminar flow hoods can be DIY or replaced with still air boxes (though less reliable).

📌 Low-Cost Alternatives for Tissue Culture