1. The Need for Transition

Plants grown in in vitro environments have:

• No exposure to wind, pests, or microbes

• Poor cuticular development

• Weak root-soil interface

• High dependence on external sugar and humidity

Without gradual exposure, they desiccate, wilt, or die when placed in standard greenhouse conditions.

2. Environmental Factors to Adjust

A controlled, stepwise shift allows plants to develop physiological defenses and adaptive structures.

3. Substrate and Potting

Use sterile or pasteurized media:

• Peat + coir + vermiculite (1:1:1)

• Avoid commercial mixes with synthetic fertilizer initially

Potting steps:

1. Gently rinse off agar (which can harbor bacteria/fungi)

2. Trim damaged roots if necessary

3. Pot into moist but not soggy substrate

4. Cover immediately with a humidity dome or clear lid

📌 Tissue Culture Potting Guide

4. Humidity Tapering

Humidity must be reduced gradually so plantlets don’t lose water too rapidly:

Each species has slightly different timing. Monitor for wilting or leaf curl as signs of too-rapid transition.

5. Preparing the Greenhouse

Before moving plants from controlled environments:

• Disinfect greenhouse tools and trays

• Clear algae and mold from misters or floors

• Use a transition shelf with partial shade

• Ensure temperature and light sensors are functional

• Group species by humidity/light needs to minimize loss

Optional: Use a quarantine bench for 3–5 days to spot infections.